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Affinity chromatography is a biochemical separation method that combines size fractionation capability of gel permeation chromatography with the ability to design a stationary phase that reversibly binds to a known subset of molecules. This method is usually used to either purify and concentrate a molecule from a mixture or to reduce the amount of a molecule in a mixture. It can also be used to exchange the composition of the medium in which a particular molecule resides.
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