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A bioreactor is a vessel in which is carried out a chemical process which involves organisms or biochemically active substances derived from such organisms.
Bioreactors are commonly cylindrical, ranging in size from some liter to cube meters,and are often made of stainless steel.
Bioreactor design
Bioreactor design is quite a complex engineering task. Under optimum conditions the microorganisms or cells will reproduce at an astounding rate. The vessel's environmental conditions like gas (i.e., air, oxygen, nitrogen, carbon dioxide) flowrates, temperature, pH and dissolved oxygen levels, and agitation speed need to be closely monitored and controlled. One bioreactor manufacturer, Broadley-James Corporation, uses vessels, sensors, controllers, and a control system, digitally networked together for their bioreactor system.
Fouling can harm the overall sterility and efficiency of the bioreactor, especially the heat exchangers. To avoid it the bioreactor must be easily cleanable and must be as smooth as possible (therefore the round shape).
Heat exchange is needed to maintain the bioprocess at a constant temperature. Biological fermentation is a major source of heat, therefore in most cases bioreactors need water refrigeration. They can be refrigerated with an external jacket or, for very large vessels, with internal coils.
Optimal oxygen transfer is perhaps the most difficult task to accomplish. Oxygen is poorly soluble in water -and even less in fermentation broths- and is relatively scarce in air (20.8%). Oxygen transfer is usually helped by agitation, that is also needed to mix nutrients and to keep the fermentation homogeneous. There are however limits to the speed of agitation, due both to high power consumption (that's proportional to the cube of the speed) and the damage to organisms due to excessive tip speed
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