Doctor Mid-Nite or Doctor Midnight is the moniker of three superheroes in the DC Comics universe. The first originates in the Golden Age of Comic Books; the others are modern creations based upon the original.

The first Doctor was Charles McNider, a surgeon. He was the earliest blind superhero, predating Marvel Comics' Daredevil. One night he was called to remove a bullet from a witness who was to testify against the mob. However, a mobster threw a grenade into the room, killing the witness and blinding McNider. McNider thought his days as a surgeon were over until one day, while he was recovering, an owl crashed through his window (remenescent of the incident that inspired Batman to dress as a bat). Taking off the bandages that were covering his eyes, he found that he could only see in perfect darkness. Using his newfound ability, he developed a special visor that let him see in light and "blackout bombs" that block out all light, and used these to fight crime. He later joined the Justice Society of America and the All-Star Squadron.

The second Doctor Midnight (note the different spelling) was Beth Chapel, an African-American woman who was McNider's student. She was blinded in an accident and discovered she now had the same night-vison as her predecessor. She joined the superhero team Infinity, Inc. She was later killed by the supervillain Eclipso.

The third Doctor Mid-Nite is Pieter Cross. He ran a free clinic in Portsmouth City, which led him to investigate a new street drug produced by an evil corporation. In retaliation, the corporation drugged him, and he got into a car accident. Like the previous Doctors, he found that he could only see in pitch dark. He too donned a costume to fight crime. The new Dr Mid-Nite carries high-tech medical equipiment in addition to his crimefighting aparatus (which includes blackout bombs). Various people he has helped aid him in his work, both as a crime-fighter and as a community surgeon, including a number of street kids. He later joined the newest incarnation of the Justice Society. He had a brief romance with his teammate, Black Canary.

Both Dr McNider and Dr Cross had trained owls named "Hooty" as "sidekicks" (Dr McNider's was the same owl who crashed through his window). The second Hooty has a mini-camera around his neck, which can feed directly to a display in Dr Mid-Nite's goggles. The goggles also enable him to see in daylight.