Seven Sisters may refer to:

• The Pleiades, seven sisters who are companions of Artemis in Greek mythology
• The Pleiades, a star cluster named for the mythological characters
• Seven Sisters, a group of oil companies
• Seven Sisters, a group of American women's colleges
• Seven Sisters, a group of seven tall Stalinist skyscrapers in Moscow, Russia.
• Qi Qiao Jie, a Chinese holiday, sometimes called "Seventh Sister's Birthday"
• Seven Sisters, England (two places):
  • Seven Sisters, Sussex, a group of chalk cliffs
  • Seven Sisters, London, an area of north London
• Seven Sister States: a region in northeastern India, comprising the states of Arunachal Pradesh, Assam, Meghalaya, Manipur, Mizoram, Nagaland, and Tripura.
• Seven Sisters, Wales, a village in the south Wales
• The Seven Sisters, fictional characters from Dungeons & Dragons
• Seven Sisters, a mountain range in British Columbia, Canada
• Seven sisters, the seven original major movie studios